If making v2 sample sheets for instruments aside from those mentioned, ensure the ultimate sample sheet has the i5 index in the right orientation as described earlier mentioned.
Recalling the creation on the PBM version, Kauffman wrote, "It wasn't lengthy just before I discovered that each issue in my own game design experienced a suspiciously comparable Resolution: 'Hey, how about if we just handle that like they did in copyright?" Awards[]
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This allows substantial figures of libraries being pooled and sequenced simultaneously during a single run. When compared to other indexing techniques, one of a kind dual indexing mitigates index hopping by filtering hopped reads.
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The MiniSeq Rapid produced a recipe adjust that takes advantage of the grafted oligo for the i5 index priming, so it really is read through from the ahead orientation comparable to a MiSeq.
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